Gene Silencers generally consist of pools of three to five target-specific 19-25 nucleotide sequences in length
For independent verification of Sigma Receptor gene silencing results, individual duplex components or plasmids are also available upon request
Suitable control antibody: Sigma Receptor Antibody (B-5): sc-137075 is recommended as control antibody for monitoring of Sigma Receptor expression knockdown by Western blotting or immunofluorescence
shRNA Plasmids generally consist of a pool of three to five lentiviral vector plasmids each encoding target-specific 19-25 nt (plus hairpin) shRNAs designed to knockdown gene expression
shRNA sequences correspond to Sigma Receptor siRNA Gene Silencer sequences
provided as transfection-ready purified plasmid DNA target-specific gene silencing
after transfection, cells stably expressing shRNAs can be isolated by puromycin selection
shRNA Plasmids are intended only for non-commercial use.
200 µl viral stock containing 1x 106 infectious units of virus (IFU) , sufficient for 10-20 transductions
Lentiviral Particles are provided as transduction-ready viral particles for gene silencing
Lentiviral Particles generally contain three to five expression constructs each encoding target-specific 19-25 nt (plus hairpin) shRNA designed to knockdown gene expression
shRNA sequences correspond to Sigma Receptor siRNA Gene Silencer sequences
After transduction, stable cell lines expressing the shRNA may be isolated via selection with puromycin
Biosafety - Lentiviral Particles are replication-incompetent and are designed to self-inactivate after transduction and integration of shRNA constructs into genomic DNA of target cells.
Lentiviral Particles are intended for non-commercial use.