MEK-4 Antibody (G-7): sc-376838

MEK-4 Antibody (G-7) is an IgG₁ κ mouse monoclonal MEK-4 antibody (also designated MKK4 antibody or MEKKK 4 antibody) that detects the MEK-4 protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. MEK-4 Antibody (G-7) is available as both the non-conjugated anti-MEK-4 antibody form, as well as multiple conjugated forms of anti-MEK-4 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor^® conjugates. A family of protein kinases located upstream of the MAP kinases and responsible for their activation has been identified. The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phosphorylates the MAP kinase regulatory threonine and tyrosine residues present in the Thr-Glu-Tyr motif of ERK. A second MEK family member, MEK-2, resembles MEK-1 in its substrate specificity. MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases. MEK-5 appears to specifically phosphorylate ERK5, whereas MEK-6 phosphorylates p38 and p38b. MEK-7 (or MKK-7) phosphorylates and activates the JNK signal transduction pathway.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

References:

1. The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product.  |  Crews, CM., et al. 1992. Science. 258: 478-80. PMID: 1411546
2. Components of a new human protein kinase signal transduction pathway.  |  Zhou, G., et al. 1995. J Biol Chem. 270: 12665-9. PMID: 7759517
3. Independent human MAP-kinase signal transduction pathways defined by MEK and MKK isoforms.  |  Dεrijard, B., et al. 1995. Science. 267: 682-5. PMID: 7839144
4. Identification and characterization of a new mammalian mitogen-activated protein kinase kinase, MKK2.  |  Wu, J., et al. 1993. Mol Cell Biol. 13: 4539-48. PMID: 8393135
5. Characterization of the structure and function of a novel MAP kinase kinase (MKK6).  |  Han, J., et al. 1996. J Biol Chem. 271: 2886-91. PMID: 8621675
6. Characterization of the structure and function of a new mitogen-activated protein kinase (p38beta).  |  Jiang, Y., et al. 1996. J Biol Chem. 271: 17920-6. PMID: 8663524
7. Mitogen-activated protein kinase kinase 7 is an activator of the c-Jun NH2-terminal kinase.  |  Tournier, C., et al. 1997. Proc Natl Acad Sci U S A. 94: 7337-42. PMID: 9207092
8. MKK7 is a stress-activated mitogen-activated protein kinase kinase functionally related to hemipterous.  |  Holland, PM., et al. 1997. J Biol Chem. 272: 24994-8. PMID: 9312105
9. Molecular cloning and characterization of human JNKK2, a novel Jun NH2-terminal kinase-specific kinase.  |  Wu, Z., et al. 1997. Mol Cell Biol. 17: 7407-16. PMID: 9372971

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MEK-4 Antibody (G-7) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-MEK-4 Antibody (G-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MEK-4 Antibody (G-7) Specifications

Alternate Product Name

MEK-4

Product Clone Name

G-7

Classification

Primary Antibody

Clonality

Monoclonal Antibody

Antibody Isotype

IgG₁ kappa light chain

Amino Acids

43-79

Epitope Target

MEK-4

Epitope Species

mouse

Product Reactivity/Detection

MEK-4

Species Reactivity/Detection

mouse, rat, human

Additional Mammalian Reactivity

canine

Additional Non-Mammalian Reactivity

N/A

Applications

WB, IP, IF, IHC(P), ELISA

Vialing Information

Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations

3 citations in various scientific publications

Recommended Secondary Detection Reagents

N/A

Available Conjugates/Forms

AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin