Method of reusing L-cystine mother liquor of enzymatic reaction

[0033] The present embodiment selects D001 type cation exchange resin as working resin, provides the pretreatment method of this resin, specifically as follows:

[0034] Take the D001 type cation exchange resin as the working resin, soak it in a saturated NaCl solution 3 times the volume of the resin for 18 hours, then rinse it with clean water, then soak it with 3% NaoH solution for 3 hours, and still rinse it with clean water until neutral. Finally, use 5% HCl solution, soak for 6 hours, rinse with water until neutral and set aside.

[0035] The reutilization method of the enzymatic reaction L-cystine mother liquor provided in this embodiment comprises the following steps:

[0036] Pack the treated ion-exchange resin into the 80L chromatographic column;

[0037] The concentration of L-cystine mother liquor starting solution was detected by HPLC, and the concentrations of L-cystine and DL-ATC were 0.96g / L and 0.78g / L respectively;

[0038] The L-cystine mother liquor was ev...

[0046] The present embodiment selects 732 sodium type cation exchange resins as working resin, provides the pretreatment method of this resin, specifically as follows:

[0047] Take 732 sodium type cation exchange resin as the working resin, soak it in a saturated NaCl solution 3 times the volume of the resin for 18 hours, then rinse it with clean water, then soak it with 3% NaoH solution for 3 hours, and still rinse it with clean water until neutral , and finally soaked in 5% HCl solution for 6 hours, rinsed with clean water until neutral for later use.

[0048] The reutilization method of the enzymatic reaction L-cystine mother liquor provided in this embodiment comprises the following steps:

[0049] Pack the treated ion-exchange resin into the 80L chromatographic column;

[0050] The concentrations of L-cystine and DL-ATC detected by HPLC were 0.96g / L and 0.78g / L respectively;

[0051] The L-cystine mother liquor was evaporated and concentrated about 10 times with MVR equi...

[0059] The present embodiment selects 732 sodium type cation exchange resins as working resin, provides the pretreatment method of this resin, specifically as follows:

[0060] Take 732 sodium type cation exchange resin as the working resin, soak it in a saturated NaCl solution 3 times the volume of the resin for 18 hours, then rinse it with clean water, then soak it with 3% NaoH solution for 3 hours, and still rinse it with clean water until neutral , and finally soaked in 5% HCl solution for 6 hours, rinsed with clean water until neutral for later use.

[0061] The reutilization method of the enzymatic reaction L-cystine mother liquor provided in this embodiment comprises the following steps:

[0062] Pack the treated ion-exchange resin into the 80L chromatographic column;

[0063] The concentrations of L-cystine and DL-ATC detected by HPLC were 0.96g / L and 0.78g / L respectively;

[0064] The L-cystine mother liquor was evaporated and concentrated about 10 times with MVR eq...